Literature summary extracted from
Boos, D.; Kuffer, C.; Lenobel, R.; Koerner, R.; Stemmann, O.
Phosphorylation-dependent binding of cyclin B1 to a Cdc6-like domain of human separase (2008), J. Biol. Chem., 283, 816-823.
Protein Variants
EC Number |
Protein Variants |
Comment |
Organism |
---|
3.4.22.49 |
S1126A |
a doxycycline-induced overexpression of S1126A mutant in HEK-293 cells leads to an increase of the G2/M cell population from 24 to 49% within 24 h compared to wild-type. Giemsa-stained prometaphase-chromosomes from S1126A expressing cells are mostly separated (61%), whereas those from wild-type or T1346E/T1363E/S1399D expressing cells are almost always paired (78 and 83%, respectively) |
Homo sapiens |
3.4.22.49 |
T1346A/T1363A/S1399A |
this triple mutant binds relatively more Cdk1 than S1126A but its Cdk1 binding capacity is much decreased compared to wildtype. A doxycycline-induced overexpression of the triple mutant in HEK-293 cells leads to an increase of the G2/M cell population from 24 to 37% within 24 h compared to wild-type. Giemsa-stained prometaphase-chromosomes from triple mutant expressing cells are mostly separated (61%), whereas those from wild-type or T1346E/T1363E/S1399D expressing cells are almost always paired (78 and 83%, respectively) |
Homo sapiens |
3.4.22.49 |
T1346A/T1363A/S1399D |
the mitotic arrest phenotype caused by the T1346A/T1363A/S1399A triple mutant is largely abrogated when just residue 1399 is changed to aspartate. Thus among the phosphorylation sites within the Cdc6-like domain serine 1399 is sufficient to support Cdk1-dependent inhibition of separase |
Homo sapiens |
3.4.22.49 |
T1346E/T1363E/S1399D |
changing the phosphorylation sites within the Cdc6-like domain to acidic residues results in a separase that can still be inhibited by Cdk1 in vivo |
Homo sapiens |
3.4.22.49 |
T1346E/T1363E/S1399S |
the mitotic arrest phenotype caused by the T1346A/T1363A/S1399A triple mutant is largely abrogated when just residue 1399 is changed to serine. Thus among the phosphorylation sites within the Cdc6-like domain serine 1399 is sufficient to support Cdk1-dependent inhibition of separase |
Homo sapiens |
Inhibitors
EC Number |
Inhibitors |
Comment |
Organism |
Structure |
---|
3.4.22.49 |
Cdk1 kinase |
phosphorylation-dependent binding of the cyclin B1 subunit of Cdk1 kinase to a Cdc6-like domain of separase inhibits separase |
Homo sapiens |
|
3.4.22.49 |
securin |
- |
Homo sapiens |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
3.4.22.49 |
Homo sapiens |
- |
- |
- |
Posttranslational Modification
EC Number |
Posttranslational Modification |
Comment |
Organism |
---|
3.4.22.49 |
phosphoprotein |
three phoshorylation sites within the the Cdc6-like domain of separase are identified which are critical for Cdk1 binding: Thr 1346, Thr 1363 and Ser 1399 |
Homo sapiens |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
3.4.22.49 |
separase is expressed as a GST- or myc-tagged fusion protein in Escherichia coli BL-21 and HEK-293 cells |
Homo sapiens |
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
3.4.22.49 |
separase |
- |
Homo sapiens |